Recent Advancements in Enzyme-Based Lateral Flow Immunoassays

Preface: Recent advancements in urban flow research

Analytical sensitivity limits for lateral flow immunoassays.

A plethora of literature describes the use of lateral flow immunoassays (LFIA). Only a fraction of that contains information about possible analytical sensitivity or limit of detection. Accordingly, we have undertaken a comprehensive survey of the literature in PubMed and present here that fraction that has information on limits of detection or analytical sensitivity. Further detail of the sear...

Recent Advances in Nanoparticles-based Lateral Flow Biosensors

Lateral flow biosensors (LFBs) provide advantages in low cost, simplicity, rapidity, stability and portability, thus making LFBs popular in biomedical, agriculture, food and environmental sciences. This article reviews recent advances of LFBs for bioassays, including the investigation of the improvements achieved by signal-amplification strategies, the application of new nanoparticle labels, no...

Silane-dextran chemistry on lateral flow polymer chips for immunoassays.

The prognosis for patients suffering from cardiovascular and many other diseases can be substantially improved if diagnosed at an early stage. High performance diagnostic testing using disposable microfluidic chips can provide a platform for realizing this vision. Amic AB (Uppsala, Sweden) has developed a new microfluidic test chip for sandwich immunoassays fabricated by injection molding of th...

Simple paper architecture modifications lead to enhanced sensitivity in nanoparticle based lateral flow immunoassays.

Lateral flow immunoassays (LFIA) are ideal biosensors to detect proteins, but their lack of sensitivity hinders their extensive use. We report a strategy that yields up to an 8-fold improvement in the sensitivity of a gold nanoparticles-based LFIA by changing the sizes of the pads. Theoretical flow simulations of the developed LFIA architectures are in accordance with the experimental results.